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    The Research Technician will assist members of the Neuroblastoma Program in planning and carrying out experiments, and taking responsibility for laboratory maintenance. Responsibilities include, but are not limited to: in vitro cell culture using sterile techniques, radioimmunoassays, Chromium release assays, ELISA, immunostaining tissue sections, immunofluorescence; PAGE, protein blotting, protein purification by Column chromatography; DNA, RNA, and protein extraction from tissues, Northern …
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    The position requires familiarity with the principles behind basic cell culture and molecular biology techniques, as well as working with mice. This includes some laboratory experience in culturing primary/established cell lines derived from human/animals. The candidate should also be familiar with principles behind performing assays of enzymatic activity and apoptosis, as well as histological analysis of tissue sections. Candidates will be expected to assist in generating tumor xenografts …
    www.nature.com   ...Read On

    Hypoxia in solid tumors is associated with treatment resistance and increased metastatic potential. Although hypoxia has been reported in pancreatic cancer patients, there is little direct evidence that this contributes to their overall poor prognosis. To address this, we examined the associations between hypoxia and biological aggression in a series of patient-derived xenografts grown orthotopically. Early passage xenografts were established from 16 patients undergoing surgery for pancreatic cancer and maintained in the pancreas of immune-deprived mice. Hypoxic cells were labeled using the 2-nitroimidazole probe EF5 and stained for immunofluorescence microscopy of tissue sections or as cell suspensions for flow cytometry. Bromodeoxyuridine (BrdUrd) uptake, microvessel density, cleaved caspase-3, and the differentiation markers E-cadherin, cytokeratin 19, and vimentin were analyzed in relation to hypoxia. Orthotopic implants closely resembled the histology of the original surgical samples. The 16 primary xenografts showed a wide range in their growth rates and metastatic potential, reminiscent of the spectrum of behavior seen in the clinic. EF5 labeling, tumor growth rates, and metastatic patterns were highly consistent within replicates, indicating a significant transmissible (genetic or epigenetic) component. Hypoxia was highly correlated with rapid tumor growth, increased BrdUrd uptake, and with spontaneous metastasis formation. mRNA expression analysis showed increased expression of genes involved in cell survival and proliferation in the hypoxic models. The results suggest that hypoxia is a major adverse prognostic factor in pancreatic cancer patients and support the introduction of techniques to measure hypoxia directly in patients and the development of treatment protocols to target hypoxia. Cancer Res; 71(8); 3110–20. ©2011 AACR.

    cancerres.aacrjournals.org


      ...Read On

    Hypoxia in solid tumors is associated with treatment resistance and increased metastatic potential. Although hypoxia has been reported in pancreatic cancer patients, there is little direct evidence that this contributes to their overall poor prognosis. To address this, we examined the associations between hypoxia and biological aggression in a series of patient-derived xenografts grown orthotopically. Early passage xenografts were established from 16 patients undergoing surgery for pancreatic cancer and maintained in the pancreas of immune-deprived mice. Hypoxic cells were labeled using the 2-nitroimidazole probe EF5 and stained for immunofluorescence microscopy of tissue sections or as cell suspensions for flow cytometry. Bromodeoxyuridine (BrdUrd) uptake, microvessel density, cleaved caspase-3, and the differentiation markers E-cadherin, cytokeratin 19, and vimentin were analyzed in relation to hypoxia. Orthotopic implants closely resembled the histology of the original surgical samples. The 16 primary xenografts showed a wide range in their growth rates and metastatic potential, reminiscent of the spectrum of behavior seen in the clinic. EF5 labeling, tumor growth rates, and metastatic patterns were highly consistent within replicates, indicating a significant transmissible (genetic or epigenetic) component. Hypoxia was highly correlated with rapid tumor growth, increased BrdUrd uptake, and with spontaneous metastasis formation. mRNA expression analysis showed increased expression of genes involved in cell survival and proliferation in the hypoxic models. The results suggest that hypoxia is a major adverse prognostic factor in pancreatic cancer patients and support the introduction of techniques to measure hypoxia directly in patients and the development of treatment protocols to target hypoxia. Cancer Res; 71(8); 3110–20. ©2011 AACR.
    cancerres.aacrjournals.org   ...Read On

    We are looking for a research technician to join our developmental neurobiology lab. Familiarity with molecular biology techniques is essential. Work will include molecular biology to subclone plasmids and genotyping of mouse mutants. It may also involve immunofluorescent staining of mouse embryos, tissue sections, cells and primary neurons, microscopy, and biochemical analyses. In addition to providing support to various research projects, he/she will coordinate management of the laborator…
    www.nature.com   ...Read On


    		   
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